GENETIC DIVERSITY AMONG Colletotrichum sublineolum PATHOTYPES ISOLATED FROM SORGHUM (Sorghum bicolor)

JOSÉ EDSON FONTES FIGUEIREDO, HENRIQUE CESTARI DEPAOLI, VINÍCIO TADEU DA SILVA COELHO, CARLOS ROBERTO CASELA, ALEXANDRE DA SILVA FERREIRA, CLÁUDIA TEIXEIRA GUIMARÃES, ELIANE APARECIDA GOMES, WELLINGTON BRESSAN

Resumo


Sorghum anthracnose, caused by Colletotrichum sublineolum is a serious disease in Brazil. The fungus is highly variable pathogenically with many physiological races. The present study was made to evaluate the usefulness of molecular methods (SDS-PAGE, RAPD, ARDRA and rDNA sequencing) for identifying C. sublineolum pathotypes isolated from sorghum. Although protein profile (SDS-PAGE) showed a relative low level of polymorphism, differences could be observed due to presence/absence of some polypeptides as well as to intensity of some bands among pathotypes. DNA profile of arbitrarily amplified sequences (RAPD) using sixteen random primers revealed a high degree of polymorphism while amplified rDNA (ITS region and 18S gene) digested with six different restriction enzymes (ARDRA) showed similar patterns. Nucleotide sequencing analysis of the complete ITS region and partial sequencing of 18S rRNA gene were useful for revealing genetic differences among the five pathotypes. In the present study, RAPD and rDNA sequencing were the most profitable methods for identifying C. sublineolum pathotypes.

 


Palavras-chave


Colletotrichum sublineolum, Sorghum bicolor, SDS-PAGE, RAPD, ARDRA, seqüenciamento de rDNA.

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DOI: http://dx.doi.org/10.18512/1980-6477/rbms.v5n02p%25p